Guidelines for submitting user-prepared Illumina sequencing libraries to the DcGC

Standard Illumina sequencing can be requested according to the read length and throughput specifications of the systems shown below. The requested coverage is mostly application dependent and can be specified for each sample during online sample submission. Please see below the available sequencing systems and their minimal coverage per sample and capacities per FC compartment. Alternative read-lengths and indexing scenarios are possible upon request if full flowcells are requested.

Minimal coverage per sample

Minimal coverage / sample is 20 mio read pairs. In case of pools this can be reduced upon request and after negotiation. Specificities of the species, sample, or project design can influence the number of reads required. We are glad to provide guidance on sequencing needs.

FC types and their capacities

FC name Possible number of sequencing cycles 1 Number of lanes 2 Number of clusters / lane | FC [Mio] 3
SP 100, 200, 300, 500 2 375 | 750
S1 100, 200, 300 2 750 | 1.500
S2 100, 200, 300 2 1.850 | 3.700
S4 35, 200, 300 4 2.250 | 9.000
Table 1: Flowcell options on NovaSeq 6000

more information

Illumina NovaSeq Flowcell specs

minimal coverage per sample

We do only accept submissions for sequencing of full flowcells

FC types and their capacities

FC name Possible number of sequencing cycles 4 Number of clusters / FC [Mio] 5
MiSeq nano v2 300, 500 0.8 - 1
MiSeq micro v2 300 3 - 4
MiSeq v2 50, 300, 500 12 - 15
MiSeq v3 150, 600 22 - 25
Table 2: Flowcell options on MiSeq

more information

Illumina MiSeqFlowcell specs

Footnotes

  1. The cycles can be customized when running complete flowcells such as: single end sequencing with 100 bp’s or 2 x 50 bp’s etc.↩︎

  2. Lanes are compartments on the FC that are physically separated from each other.↩︎

  3. Clusters are distinct monoclonal colonies of amplified NGS libraries on the FC surface after the so-called cluster generation.↩︎

  4. The cycles can be customized when running complete flowcells such as: single end sequencing with 100 bp’s or 2 x 50 bp’s etc.↩︎

  5. Clusters are distinct monoclonal colonies of amplified NGS libraries on the FC surface after the so-called cluster generation.↩︎