Protocols

Low input samples related protocols

• Protocol for BSA coating of tubes
• Isolation of low amount of RNA with Trizol and glycogen

Plate based scSeq related protocols

• Protocol for Smart-Seq lysis buffer
• FACS pre-sort efficiency test for 384-well plates

Nuclei isolation protocols

• Suggested protocol for nuclei isolation for subsequent bulk ATAC-Seq
• 10X Genomics suggested protocol for nuclei isolation for subsequent snRNA-Seq preparation
• 10X Genomics suggested protocol for nuclei isolation for subsequent snATAC-Seq preparation